Conference Schedule
Day1: May 10, 2018
Keynote Forum
Thomas Boldicke
Helmholtz Centre for Infection Research, Germany
Title: Intrabodies knocking down intracellular cancer antigens
10:00-10:40
Biography
Abstract
Intrabodies can be used to target and knock down virtually every protein inside the cell. The knockdown of intracellular cancer antigens by intrabodies is promising. Cancer antigens passing the endoplasmatic reticulum (ER) are inactivated by ER intrabodies retained inside the ER and expressed in the single-chain variable fragment (scFv) format. Cytosolic and nuclear cancer targets are inhibited by neutralizing single domain antibodies which comprises only the variable domain of the heavy chain derived from camels or sharks. This talk will give an overview of in vivo targeting of cancer antigens by intrabodies in mouse tumor models and will demonstrate an example of ER intrabodies inhibiting the polysialyltransferases in rhabdyomasarcoma cells in a xenograft tumor mouse model.
Pascal Rihet
Inserm and Aix-Marseille University, France
Title: From genome scans to the identification of functional genetic variants associated with malaria resistance
10:40-11:20
Biography
Pascal Rihet has a long lasting experience of research in the field of genetics and genomics of infectious diseases. He has mapped malaria and sepsis predisposing genes by using genetic linkage or association approaches. Furthermore, he has identified many variants associated with the disease; most of those genetic variants are located within noncoding regions. He has provided evidence that several variants have a cis-regulatory effect, suggesting that the regulation of gene expression is critical for the pathogenesis. In this way, he has investigated gene expression profiles in patients or in mouse models, and identified a number of genes whose expression is up- or down-regulated before or at the onset of the disease. He was the Deputy Director of the TAGC laboratory. Currently he is the Director of TAGC laboratory. The research scope of the laboratory is Genetics, Genomics and Bioinformatics. He is a Professor of Genomics and Immunology at AMU.
Abstract
The contribution of host genetic factors to resistance or susceptibility to Plasmodium falciparum malaria has been widely studied. Nevertheless, a few genome scans have been performed, and few of them led to the discovery of loci significant at the genome level and to the identification of functional variants potentially causal. Here we describe loci genetically linked to malaria phenotype at the genome level and genetic variants located within those loci and associated with malaria phenotype in two independent populations. Furthermore, we provide evidence of a cis-regulatory effect of the genetic variants, suggesting that those variants are causal. We mainly focus on genes and genetic variants located within chromosome 6p21, which has been linked to mild malaria. These include TNF and NCR3, which encode a major actor of inflammation and a receptor of natural killer cells involved the cytotoxicity function, respectively. Also, the results are in line with those supporting the role of TNF in malaria on the one hand and allow us to propose a new biological model to explain the association of a cis-regulatory variant of NCR3 with mild malaria, on the other hand. Also, the genetic variation that alters the activation of natural killer cells may influence human malaria resistance.
Tracks
- Classical Immunology | Tissue Engineering and Regeneration | Immunoinformatics | Immunological Disorders | Autoimmune Diseases | Infectious Disease Drivers | Emerging and Re-emerging Infectious Diseases | Remedy for Immunity and Infection
- Lunch Break
- Networking & Refreshment Break
Location: Spessart
Biography
Abstract
As infections with Borrelia are often accompanied by coinfections, they should also be taken into consideration. coinfections complicate the diagnosis and treatment because they often evoke a more complex symptomatology. The co-pathogens can cause very specifi c, but also unspecifi c and “overlapping” symptoms. in order to better detect these pathogens, Dr. schwarzbach has developed a coinfections checklist. The analysis of the checklist enables a better targeted selection of necessary laboratory tests for coinfections. This preselection prevents expensive panel testing for our patients. our team will gladly provide you with the automated coinfections checklist and more information about our modern diagnostic test methods.
Biography
Abstract
Biography
Founded in 1997, Chemometec specialises in the design, development and production of high quality instruments using patented technology for a wide range of applications in cell counting and evaluation. Like many companies with unique technology, we had an interesting birth as talented development specialists with new ideas broke free from conservative corporate constraints.We work closely with life science companies, research institutes, universities, hospitals, specialist clinics and a wide range of food and beverage manufacturers, matching our technical development expertise to customer needs - with quality results, reliability, cost-efficiency and ease-of-operation as our guiding principles. We support a growing customer base worldwide with responsive technical services and the ready availability of a wide range of consumables.
Abstract
Location: Spessart
Hadaf Dhafir Al Yaseen
University of Baghdad, Iraq
Chair
Yawei Liu
University of Copenhagen, Denmark
Co Chair
Manijeh Vafa Hofmann
Karolinska Institutet, Sweden
Title: Detection of malaria parasites after treatment in travelers: A 12-months longitudinal study and statistical modelling analysis
Biography
Abstract
Hans Kollberg
Uppsala Universitet, Sweden
Title: Antibodies instead of antibiotics to kill bacteria - experience with anti-pseudo IgY with cystic fibrosis patients
Biography
Abstract
Location: @ Rastaurant palate friend
Huang Wei Ling
Medical Acupuncture and Pain Management Clinic, Brazil
Title: Can recurrent furunculosis be treated without the use of antibiotics?
Biography
Abstract
Desheng Hu
Wuhan Union Hospital, China
Title: Artery tertiary lymphoid organs: powerhouses of atherosclerosis immunity
Biography
Abstract
Rochelle Van der Merwe
South African Society of Travel Medicine, South Africa
Title: The Hysteria surrounding Listeria in South Africa
Biography
Abstract
Julie Di Cristofaro
Aix Marseille University, France
Title: HLA Ib molecules in alloimmunization and inflammatory response
Biography
Abstract
Location: @ Foyer
Biography
Abstract
Yawei Liu
University of Copenhagen, Denmark
Title: Neuronal IFN-beta–induced PI3K/Akt-FoxA1 signaling is essential for generation of FoxA1+ Treg cells
Biography
Abstract
Sameh Elmorsy Hassan
El-Matrya Educational Hospital, Egypt
Title: Discitis osteomyelitis pathophysiology and different management
Biography
Abstract
Day2: May 11, 2018
Keynote Forum
Huang Wei Ling
Medical Acupuncture and Pain Management Clinic, Brazil
Title: Why do patients still catch hospital infections despite the practice of infection prevention and control programs?
10:00-10:40
Biography
Abstract
Statement of the Problem: Very few publications provide sound scientific data used to determine which components are essential for infection prevention and control (IPC) programs in terms of effectiveness in reducing the risk of infection. In recent years, a range of regional best practice or policy principles have been developed that address what could be considered as core components of IPC programs. However there remains a major gap in relation to the availability of international best practice principles for core components of IPC programs.
Purpose: The purpose of this study was to show why patients still catch hospital infections despite IPC programs. A better understanding of a variety of theories is needed that could explain the physiopathology of diverse diseases described in the medical past history, which are usually disregarded clinically today. A broader view seems to show the necessity of seeing the patientas a whole; not only focusing on the disease in the prevention of these hospital infections.
Methodology: A review of these theories such as those presented by Hippocrates (Natural forces within us are the true healers of disease), as well as others from oriental medicine, which explain that diseases originate from three factors: external (exposure to cold, heat, humidity, wind and dryness), internal (emotional) and dietary.
Findings: Having a broader view of the patient as a whole (Yin, Yang, Qi, blood energy and heat retention), we can understand better the formation of hospital infection which is a systemic energy reaction of our body undergoing normal hospital treatment.
Conclusion: To understand better why a patient is still catching hospital infections, despite these IPC programs, we need to broaden our view observing all emotional, environmental and dietary factors, as well as studying the patient’s energy situation atthe moment of admittance identifying the risk of hospital infection.
Thomas Grundstrom
Umeå University, Sweden
Title: Regulation of diversification and affinity maturation of antibodies
10:40-11:20
Biography
Abstract
B-lymphocytes can modify their immunoglobulin (Ig) genes to generate antibodies with a new isotype and enhanced affinity. Activation-induced cytidine deaminase (AID) is the key mutagenic enzyme that initiates these processes. How somatic hypermutation (SH) and class switch recombination (CSR) are targeted and regulated to understand how we achieve good antibodies. The trans-acting factors mediating specific targeting of AID and thereby SH and CSR have remained elusive. How AID is recruited was still a big mystery. We show that mutant E2A transcription factor with defect inhibition by the Ca2+ sensor protein calmodulin results in reduced B cell receptor (BCR), IL4- plus CD40 ligand-stimulated CSR to IgE. AID is shown to be together with the transcription factors E2A, PAX5 and IRF4 in a complex on key sequences of the Igh locus in activated mouse splenic B cells. Calmodulin shows proximity with them after BCR stimulation. Direct protein-protein interactions are shown to enable formation of the complex. BCR signaling reduces binding of the proteins to some of the target sites on the Igh locus, and calmodulin resistance of E2A blocks this reduction. Thus, E2A, AID, PAX5 and IRF4 are components of a CSR and SH complex that calmodulin binding redistributes on the Igh locus. We present also that initiation of antibody diversification leads to formation of a mutasome, a complex between many proteins that enable repair at high error rate of the uracils made by AID on Ig genes but not on most other genes. We show also that BCR activation, which signals end of successful SH, reduces interactions between some proteins in the complex and increases other interactions in the complex with varying kinetics. Furthermore, we show increased localization of SH and CSR coupled proteins on switch regions of the Igh locus upon SH/CSR and that BCR signaling differentially change the localization.
Sasha Berdichevski
University of Cambridge, UK
Title: Designing scaffolds for tissue engineering: 3D geometry-function relationship
Biography
Sasha Berdichevski is a Post-doctoral Research Associate in Engineering Department at University of Cambridge, UK. She has obtained a Blavatnik Fellowship by the Blavatnik Family Foundation, British Council and University of Cambridge, and currently she holds a Marie Curie Fellowship. She has been awarded as outstanding Researcher in Engineering and Science Award and Prize for Excellence in Nano-science and Nanotechnology during her PhD in the Technion, Israel. She has published her research in leading journal papers, and co-authored publications in three books. Her research interests include “Biomaterials, tissue engineering, scaffold-tissue/cell interactions, and scaffolds’ 3D geometry function relationship”.
Abstract
One of the main goals in producing engineered tissues at clinically relevant dimensions is creating perusable vascular networks, since cell viability and function cannot be sustained through diffusion alone. Therefore a great deal of research in the field of regenerative medicine has been devoted to establish in vitro pre-vascularization approaches. In this context, we propose to create capillary-like networks using human umbilical cord endothelial cells, cultured with human osteoblasts, as these cells were demonstrated to have both direct and indirect pro-vasculogenic effects, within freeze-dried collagen scaffolds with tailored pore architecture. We guided scaffold pore architecture by manipulation of the freeze-drying conditions; producing porous scaffolds with randomly oriented (isotropic) or uniaxially aligned (anisotropic) pore architectures. We characterized the scaffolds’ structural, permeability and mechanical properties and showed that pore architecture affected the invasion, morphology and self-organization of the endothelial cells, in both mono- and co-cultures. Results showed that cell proliferation and metabolic activity were affected by pore architecture as well. Pore anisotropy promoted more uniform cell infiltration deeper within the scaffold, and improved cell organization into multi-cellular vessel-like networks. Co-culture conditions further improved the network quality. We suggest that deeper cell infiltration, along with more efficient medium perfusion within the anisotropic scaffolds account for these findings. However, the exact mechanism and conditions for optimal 3D vascular network formation as function of pore architecture have yet to be established.
12:20-13:00
Biography
Roman Bauer is an MRC Research Fellow at Institute of Neuroscience-Newcastle University, with joint affiliation with the School of Computing. His research involves computational models to better understand how neural tissue evolves during development. He received his Bachelor’s and Master’s Degree in Computational Science and Engineering from ETH Zurich, Switzerland. Afterwards, he did his Doctoral studies at Institute for Neuroinformatics (INI)- ETH and University Zurich, working on simulations of cortical development. After Postdoctoral work at Newcastle University from 2013 to 2016, he took up a prestigious MRC fellowship. His research interests include “Neural development, neural degeneration, neural disorders, gene regulatory networks and cryopreservation”.
Abstract
Biological tissue often exhibits extraordinary complexity. For example, neural tissue comprises large numbers of neurons with cell-type specific axonal and dendritic arborisation, highly structured synaptic connectivity, and fine-tuned electrical activity. A better understanding of how such tissue complexity develops is often essential for tissue engineering purposes. To this end, author will present some of his computational models of neural tissue development, demonstrating how complex structure and function can be generated based solely on simple genetic rules. These multi-scale models comprise intracellular as well as extracellular dynamics in a detailed, physical 3D environment. In particular, author will elaborate on computational models of cortical and retinal structure and function, ranging across different spatial scales. By modelling the biological self-organization of such tissue, predictions are made and so novel hypotheses are generated, which can be experimentally validated. Moreover, these models can inform and guide tissue engineering protocols. Finally, author will discuss modern computational approaches, including the BioDynamo software, which is a collaborative project with project partner CERN Openlab. Overall, author will emphasize the importance of computer models as a tool to advance tissue engineering approaches.
Tracks
- Clinical Immunology | Tissue Engineering and Regeneration | Immunological Variability | Immunity and Host defence | Immune Adverse Effects | Microbiology and Clinical Infections | Versatile Immunology | Study of Evolution
- Lunch Break
- Networking and Refreshments Break
- Poster Presentation
Location: Spessart
Hans Kollberg
Uppsala Universitet, Sweden
Chair
Manijeh Vafa Hofmann
Karolinska Institute, Sweden
Co Chair
Aarti Sharma
Ayurvedic Physician, Govindrakshak Ayurvedic and Acupuncture Centre, India
Title: Ayurveda a ray of hope for autoimmune disease psoriasis: A case study
Biography
Abstract
An autoimmune disease condition arises due to abnormal immune system response. Psoriasis is one of very common auto immune disease. Case presented here is of thirty two year old male patient who have been suffering from psoriasis since last ten years. He had red itchy patches on both limbs and scalp with white silvery scales on its top. Ayurvedic treatment was started for his psoriasis, in which he was given purification therapy procedure known as Vamana followed by Takradhara for 21 days. Along-with, this he was given Mahatiktaka ghrit 20 ml with Mahamanjishtadi Kashaya 40 ml twice a day during Takradhara procedure. During Takradhara he was also given Virechana, another purification (Shodhana therapy) with Avipathi Churna. In continuation of this treatment he was given Talapodichhil (panchkarma procedure) treatment for another fourteen days along with Rasayana (rejuvenate) therapy with the herb Plumbago zeylanica. After these panchkarma procedures patient was given mahamanjishtadi kashayam 40ml and kaishore guggulu two tablets twice day for six months. With above treatment patient showed relief in all clinical parameters, particularly his itching and silvery scales disappeared. After 6 months, it was found that Ayurvedic treatment showed improvement in both Psoriasis Area and Severity Index (PASI) score along with Dermatological Life Quality Index (DLQI). The above case study shows that Ayurveda is a ray of hope for such chronic autoimmune disease like psoriasis and further extensive studies should be carried out for such treatment modalities which may prove very beneficial for people suffering from psoriasis.
Location: Rastaurant palate friend
Thomas Boldicke Helmholtz
Centre for Infection Research, Germany
Title: ER - intrabody mediates knockdown of mouse IFN alpha in macrophages and dendritic cells
Biography
Abstract
Katja Bettina Ferenz
University Hospital Essen, Germany
Title: Functionality of perfluorodecalin-based artificial oxygen carriers: impact on the whole organism and on cellular level
Biography
Abstract
Huang Wei Ling
Medical Acupuncture and Pain Management Clinic, Brazil
Title: Can autoimmune hepatitis be treated without the use of corticosteroids and immunosuppressive drugs?
Biography
Abstract
Özlem Vural
Technical University of Berlin, Germany
Title: Generating a 3D human thyroid model in vitro
Biography
Abstract
Dehghani SN1, Haghani I1, Namazi, F2, Ghaderi A3
Department of Surgery (1) and pathology (2), School of Veterinary Medicine, Shiraz University, and (3) Cancer research centre, Shiraz University of Medical science, Iran
Title: Induction of liver cirrhosis and treatment of cirrhotic liver by mesenchymal stem cells derived from adipose tissue in rat model
Biography
Abstract
Liviu Steier
University of Warwick, Germany
Title: Tissue regeneration and maintenance protocols for periimplant mucositis and implantitis
Biography
Abstract
Location: Foyer
David R Thomas
Lyme disease Advocate, New York
Title: The perils of invisible disease and Lyme related issues
Biography
Abstract
Location: Spessart
Yu-zhen Tan, Qiang-li Wang, Hai-jie Wang and Yong-li Wang
Shanghai Medical School of Fudan University, China
Title: Cardiac patch loading MSCs overexpressing Tβ4 promotes repair of the infarcted myocardium by endogenous regenerative mechanisms
Biography
Abstract
Recent studies suggest that the epicardium plays an important role in cardiomyogenesis during development, while it becomes quiescent in adult heart. Thymosin beta 4 (Tβ4) has an effect on activating the epicardium. However, effectiveness of Tβ4 administration is unsatisfactory. Therefore, this study prepared cardiac patch and investigated efficiency of activating the epicardium by Tβ4 released sustainedly from the cardiac patch. Mesenchymal stem cells (MSCs) isolated from bone marrow of rats and mice were transfected with Tβ4. Tβ4 release from the cells was determined with an acquity ultra-performance liquid chromatography system. For preparing of cardiac patch, the cells transfected with Tβ4 and Flag were seeded on PLACL/collagen membrane formulated by electrospinning. The survival and proliferation of the cells on the nanofibers were examined after treatment with hypoxia. In MI models of rats and Wt1CreERT2/+, R26mTmG mice, the patches were implanted on the epicardium of the infarcted region. In rat models, differentiation of the epicardium-derived cells (EPDCs) and the engrafted MSCs towards cardiomyocytes and vascular cells was examined by Wt1 immunostaining and Flag labeling. In transgenic mouse models, the activated EPDCs expressed GFP. At four week after implantation of the patches, cardiac function was improved significantly, scar area in the infarcted region was reduced obviously. EPDCs increased in subepicardium and myocardium, and some Wt1+ cells and GFP+ cells expressed CD31, α-SMA or cTnT. Moreover, c-kit+ cells were observed in subepicardium and myocardium, and a few of them expressed CD31, α SMA or cTnT. Flag labeling showed that some engrafted MSCs migrated into subepicardium and myocardium. These results suggest that Tβ4 released from the transfected MSCs in PLACL/collagen nanofibrous patches may effectively attenuate left ventricular remodeling and improve cardiac function by activating the epicardial cells and recruiting endogenous stem cells. Our finding provides a novel strategy for myocardial regeneration by enhancing the endogenous regenerative mechanisms.
Hai jie Wang, Hai-feng Zhang, Yu-zhen Tan and Yong-li Wang
Shanghai Medical School of Fudan University, China
Title: Lymphatic endothelial progenitor cells and VEGF-C loaded with self-assembling peptide nanofibers promote lymphangiogenesis in infarcted myocardium
Biography
Abstract
Mina Tadjalli1, Giti Zarinfard1 and Shahnaz Razavi2
1School of Veterinary Medicine, Shiraz University, Iran 2School of Medicine, Isfahan University of Medical Sciences, Iran
Title: Effect of laminin on neurotrophic factors expression in schwann-like cells induced from human adipose-derived stem cells in vitro
Biography
Abstract
Aditi Singh
Amity University, India
Title: Inflammatory mechanism during Japanese encephalitis virus infection
Biography
Abstract
Arnav Gupta
Biomedical Research Institute of New Jersey, USA
Title: Inhibitory effects of dipeptide analogue, DAPT, on γ-secretase causing decrease in amyloid-β concentration in neuroblastoma cells
Biography
Abstract
Hans Kollberg
Uppsala University, Sweden
Title: More than 22 years of clinical studies on anti-pseudomonas IgY to cystic fibrosis patients
17:10-18:00
Biography
Abstract
Rochelle Van der Merwe
South African Society of Travel Medicine, South Africa
Title: Anopheles…the anihalator
Biography
Abstract
Yawei Liu
BRIC - University of Copenhagen, Denmark
Title: Neuronal IFN-beta–induced PI3K/Akt-FoxA1 signaling is essential for generation of FoxA1+Treg cells
Biography
Abstract
Hadaf Dhafir El Yassin1 and Rana A Hadi2
1University of Baghdad, Iraq 2Iraqi Board for Medical Specializations, Iraq
Title: Anti smooth muscle antibodies (ASMA) and tumor necrosis factor (TNF) in Iraqi patients infected with hepatitis C virus
Biography
Abstract
Yosra Bouraoui1, 2, N Ben Rais3 and R Oueslati1
1Carthage University, Tunisia 2Jendouba University, Tunisia 3HMPIT, Tunisia
Title: The immune profile of prostate epithelial cells by IL-6 activation mediated by STAT3 and AKT signaling pathways
Biography
Abstract
Alexandra Scheer1, Anna Wrobeln2, Michael Kirsch2 and Katja B Ferenz2
1University Hospital Essen,Germany 2University of Duisburg-Essen,Germany