Stem Cell Bioprocessing

Bioprocess Technology   process living matter specific optimal conditions to make a required product. Stem-cell bioprocessing is the use of stem cells to treat or prevent a disease or condition in order to preserve the cell functionality.  Bone marrow transplant is the most preferably used stem-cell therapy. The major or fundamental drawback is the lack of real-time, on-line, in-situ, quantitative information with respect to cellular behaviour in tissue culture. Therefore the control, optimisation and scale-up of stem cell bioprocesses are essentially empirical, which results in    inadequate cell expansion and decreased product quality   with inconsistent cell phenotypes. To harness the immense potential of stem cells (SCS) in terms of their plasticity ,replication and expansion capabilities, the physiological activity in relation to the environmental culture parameters  such as pH, dissolved oxygen, nutrients/metabolite concentrations and growth factor concentrations needs to be observed quantitatively with the needed level of accuracy and subsequently evaluated in a biologically  manner. The systematic development of clinically relevant culture systems and methodologies control and regulate stem cell self-renewal, expansion, differentiation, and death.